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RESEARCH PAPERS        

                                                                                  

  1. Chenxi Wang, Xinyu Feng, Jun Feng, Hui Chen, Tianmu Chen, Kun Yin; Advances in the diagnosis and genomic research of surveillance-response activities in emerging, re-emerging, and unidentified infectious diseases; Frontiers in Public Health; 2023. 11, 1182918

  2. Hui Chen, Sungwan Kim, Joseph Michael Hardie, Manoj Kumar Kanakasabapathy, Supriya Gharpure, Prudhvi Thirumalaraju, Sahar Rostamian, Qingsong Lei, Srisruthi Udayakumar, Gi Won Cho, and Hadi Shafiee; Deep learning-assisted sensitive detection of fentanyl using a bubbling-microchip; Lab on a Chip, 2022,22, 4531-4540.

  3. Yi Xie, Huimin Li, Fumin Chen, Srisruthi Udayakumar, Khyati Arora, Hui Chen, Yang Lan, Qinqin Hu, Xiaonong Zhou, Xiaokui Guo, Leshan Xiu, Kun Yin;Clustered Regularly Interspaced short palindromic repeats-Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives. Advanced Science, 2022, 2204172.

  4. Hui Chen, Sheng Feng, Wan Zhou, Zhao Li, Melissa Richard-Greenblatt and Ping Wang; Pretreatment Methods for Human Nasopharyngeal Swabs to Increase Signal to Noise Ratio of High Sensitivity Immunoassays; ACS Measurement Science Au; 2022, 2, 5, 414–421.

  5. Hui Chen, Zhao Li (co-first author), Sheng Feng, Anni Wang, Melissa Richard-Greenblatt, Emily Hutson, Stefen Andrianus, Laurel J. Glaser, Kyle G. Rodino, Jianing Qian, Dinesh Jayaraman, Ronald G. Collman, Jae Seung Lee, Sara Cherry, Alejandra Fausto, Susan R. Weiss, Hyun Koo, Patricia M. Corby, Ping Wang; Femtomolar SARS-CoV-2 Antigen Detection Using the Microbubbling Digital Assay with Smartphone Readout Enables Antigen Burden Quantitation and Tracking; Clinical Chemistry, Volume 68, Issue 1, 2022, Pages 230–239,.

  6. Hui Chen, Zhao Li (co-first author), Sheng Feng, Keng-Ku Liu, Ping Wang; Development and Clinical Validation of a Sensitive Lateral Flow Assay for Rapid Urine Fentanyl Screening in the Emergency Department. Clinical Chemistry 2020, 66 (2), 324-332

  7. Hui Chen, Dimple Chavan (co-first author), Mary Crum, Binh Vu, Mohammad Safari, Maxwell Smith, Peter Vekilov, Jacinta C. Conrad, Katerina Kourentzi, Richard C. Willson; Neutral DNA–avidin nanoparticles as ultrasensitive reporters in immuno-PCR; Analyst; 2020, 145, 4942.

  8. Hui Chen, Zhao Li, Lingzhi Zhang, Philippe Sawaya, Jianbo Shi, Ping Wang; Quantitation of Femtomolar-Level Protein Biomarkers Using a Simple Microbubbling Digital Assay and Bright-Field Smartphone; Angewandte Chemie International Edition; 2019, 131(39), 14060-14066.

  9. Hui Chen, Keng-Ku Liu, Zhao Li, Ping Wang; Point of care testing for infectious diseases. Clinica Chimica Acta; 2019, 493, 138-147

  10. Hui Chen, Zhao Li (co-first author), Ping Wang; Lateral Flow Assay Ruler for Instrument-free Quantitative and Rapid Point-of-care Testing; Analyst; 2019, 144 (10), 3314-3322.

  11. Hui Chen, Mary Crum, Dimple Chavan, Binh Vu, Katerina Kourentzi, Richard C. Willson; Nanoparticle based Proximity Ligation Assay for Ultrasensitive, Quantitative Detection of Protein Biomarkers；ACS Applied Materials & Interfaces; 2018 10(38): 31845-31849.

  12.  Hui Chen, Hagström A. E. V., Kim, J., Garvey, G., Paterson, A., Ruiz-Ruiz, F., Raja, B., Strych, U., Rito-Palomares, M.; Kourentzi, K., Conrad, J. C.; Atmar, R. L., Willson, R. C.; Flotation Immunoassay: Masking the Signal from Free Reporters in Sandwich    Immunoassays. Scientific Reports; 2016, 6, 24297

  13. Hui Chen, Yang Zeng, Wei Liu, Shan Zhao, Jinhui Wu, Yanan Du, Multifaceted applications of nanomaterials in cell engineering and therapy, Biotechnology Advances; Volume 31, Issue 5, 2013.

  14. Hui Chen, Yulan Wang, Shiping Xu, Jianchen Wang; Influence of High Frequency Ex-electric Field on Etching Process and Shape of Pores for Nuclear Track Film. Atomic Energy Science and Technology, vol.45, No. 7, p853, 2011.

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BOOK CHAPTERS                                                                  

  1. Hui Chen, Qingsong Lei, Gaojian Li, Srisruthi Udayakumar, Joseph M. Hardie, Hadi Shafiee; At-Home Sperm Banking Kits; In “Industrial breakthroughs in cryopreservation and cryo-storage”; Edited by Peter Nagy, Alex Varghese, Mississauga, Ashok Agarwal; Springer Nature, 2022.

  2. Hui Chen, Xiaokang Li, Yanan Du; ‘1D~3D Nano-Engineered Biomaterials for Biomedical Applications’; In “Integrated Biomaterials for Medical Applications”; Edited by Murugan Ramalingam and Ashutosh Tiwari; VBRI Press, 2012.

 

PATENTS      

                                                                                        

  1. Hui Chen, Ping Wang; Rapid and Ultrasensitive SARS-CoV-2 Antigen Detection for Acute Infection Screening in Community Settings; U.S. Provisional Application No.: 63/035,129 filed on June 05, 2020.

  2. Hui Chen, Zhao Li, Keng-Ku Liu, Ping Wang; Devices and Methods for Rapid Screening of Drugs of Abuse and Other Analytes; U.S. Provisional Application No.: 62/874,643 filed on July 16, 2019.

  3. Hui Chen, Zhao Li, Ping Wang; Point-Of-Care Diagnosis System and Methods; U.S. Provisional Application No.:  62/730,719; 2018.

  4. Hui Chen, Mary Crum, Dimple Chavan, Ekaterini Kourentzi, Binh Vu, Richard Willson; Highly-Specific Assays, UHID 2016-051; 2016

Quantitation of femtomolar‐level protein biomarkers using a simple microbubbling digital assay and bright‐field smartphone imaging

Quantitating ultra-low concentrations of protein biomarkers is critical for early disease diagnosis and treatment. However, most current point-of-care (POC) assays are limited in sensitivity. Herein, we introduce an ultra-sensitive and facile microbubbling assay for the quantification of protein biomarkers with a digital-readout method that requires only a smartphone camera. We used machine learning to develop a smartphone application for automated image analysis to facilitate accurate and robust counting. Using this method, post-prostatectomy surveillance of prostate specific antigen (PSA) can be achieved with a detection limit (LOD) of 2.1 fM (0.060 pg/mL), and early pregnancy detection using βhCG can be achieved with a of 0.034 mIU /mL (2.84 pg/mL). This work provides the proof-of-principle of the microbubbling assay with a digital readout as an ultra-sensitive technology with minimal requirement for power and accessories, facilitating future POC applications.

Clustered Regularly Interspaced short palindromic repeats‐Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives

Mitigating the spread of global infectious diseases requires rapid and accurate diagnostic tools. Conventional diagnostic techniques for infectious diseases typically require sophisticated equipment and are time consuming. Emerging clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) detection systems have shown remarkable potential as next-generation diagnostic tools to achieve rapid, sensitive, specific, and field-deployable diagnoses of infectious diseases, based on state-of-the-art microfluidic platforms. Therefore, a review of recent advances in CRISPR-based microfluidic systems for infectious diseases diagnosis is urgently required. This review highlights the mechanisms of CRISPR/Cas biosensing and cutting-edge microfluidic devices including paper, digital, and integrated wearable platforms. Strategies to simplify sample pretreatment, improve diagnostic performance, and achieve integrated detection are discussed. Current challenges and future perspectives contributing to the development of more effective CRISPR-based microfluidic diagnostic systems are also proposed.

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